Location : IC: NIA Street: 9000 Rockville Pike Bldg: T44 Room: 100 City: Bethesda State & Zip: MD 20814

Weekly Hours - PT: Less than 30 hours per week Total No. of Hours : 10

Overall Position Summary and Objectives

Under this task order, the contractor will independently provide support services to satisfy the overall operational objectives of the National Institute on Aging Center for Alzheimer's and Related Dementias (CARD). The primary objective is to provide services and deliverables through performance of support services.

Min Education - Master's

Resume Max Pages - 3

ADDITIONAL QUALIFICATIONS

Certifications & Licenses

• Master's degree in a cell biology, neurobiology, or related discipline

Skills (Ranked By Priority)

1. Knowledge of proper laboratory disposal methods and procedures
2. Minimum of three (3) years of related experience
3. Strong communications skills, both oral and written
4. Hands-on experience with cell culture in particular working with iPS cells, design, implement and troubleshooting.
5. Excellent analytical, organizational and time management skills

1, 2, 3, 4, 5 represents priority rankings, where 1 is highest priority and 5 is lowest priority of those ranked

Software

• Microsoft PowerPoint
• Excel
• Microsoft Word

Field of Study

• Biology
• Miscellaneous Biology
• Biochemical Sciences
• Neuroscience

Statement of Work Details

Provides technical experience needed to assist in studying the basic principles of plant and animal life

• Keep detailed, organized, and accurate digital and physical experimental records.
• Update and maintain laboratory notebooks and databases
• Attend meetings and present results and findings for staff discussion
• Work with staff on performing large-scale automated iPSC cell culture experiments
• Work with staff on performing various experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines 1
• Work with staff to characterize genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models
• Work with staff to apply published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis.
• Collect, Analyze, interpret, and organize data and present results in team and departmental meetings.
• Work independently in designing, developing, and performing new methods
• Support other research projects
• Work independently on expanding and banking mutant iPSC lines.
• Evaluate the stability of iPSC and iPSC-derived cells across multiple engineered and non-engineered lines.
• Work with staff to provide insight into the effects of ADRD-associated mutations on cellular and molecular readouts in cell culture- Keep detailed, organized and accurate digital and physical inventory from mutant iPSC lines banked. - Train Staff on cell culture techniques
• Train staff on the proper usage of equipment.
• Provide scientific and technical expertise to all visiting fellows, post-docs, and technicians internal and external.
• Support other research projects.

Assists with performing basic laboratory experiments and procedures

• Collaborate with other team members to carry out large-scale cell culture protocols using manual and high throughput robotic systems
• Perform standard immunological techniques, including western blotting and immunostaining 2
• Independently design, troubleshoot, and carry out cell culture experiments to develop iPSC differentiations- Transduce iPSCs with lentivirus and transfect iPSCs with plasmids as part of routine cell culture experiments.
• Support other research projects performing cell culture protocols manual or high throughput scale.
• Support other research projects performing mycoplasma assay of routine cell culture.

Prepares, organizes and maintains various lab samples, supplies, and equipment

• Operate laboratory equipment to analyze samples 3
• Calibrate, maintain, and troubleshoot instruments and equipment in the laboratory
• Maintain and perform staff training on scientific instrumentation, cell culture microscopes, microanalytical balances, centrifuges, automated platforms
• Operate, troubleshoot, and train others to use laboratory equipment and software- Train staff on the proper usage of laboratory instruments and equipment.
• Provide assistance to laboratory staff in designing experiments, using instrumentation, data acquisition and analysis, and preparing figures for publication.
• Review scientific equipment and resources including reagents, stocks of frozen cells, mammalian samples including acquisition, documentation, and storage
• Keep inventory of consumables and reagents use for cell culture techniques.

Learns new laboratory techniques and procedures

• Develop image analysis routines to analyze the effects of disease-associated mutations on cellular phenotypes
• Develop new differentiation protocols for iPSC lines to differentiate into disease-relevant cell types like neurons, astrocytes, and microglia.
• Learn how to operate, and implement novel methods on liquid handling platforms (Hamilton, Biotek)